Device
Ateam

Part:BBa_K1023016:Design

Designed by: Shreyans Chordia   Group: iGEM13_Wageningen_UR   (2013-09-27)


FRET based ATP biosensor for expression in Aspergillus niger


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1082
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1345


Design Notes

It has illegal restriction sites for the standard 10 assembly. The cds of the part is flanked by NdeI and NotI restriction sites.


Source

Imamura lab

References